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Division: Translational

Services

• Consultation on design and implementation of Correlative Studies for Clinical Trials
• Sample Handling (receiving, tracking, storage, distribution)
• Sample Processing (blood, tissue and paraffin block processing)
  • Routine Blood Processing (PBMC, serum, or plasma isolation)
  • DNA, RNA, protein extraction
  • Nucleic acid and protein quantitation
  • Flow or laser scanning cytometry staining
• Lab Analysis
  • Standard ELISAs (Fluorescent or Luminescent platforms)
  • MSD Multiplex Analysis (Mesoscale Electrochemiluminescence platform)
  • Comet assays
• Preclinical Drug Testing
  • Single or multiple agent cytotoxicity (MTT or DNA content assays)
  • Multiple agent analysis (Synergy analysis)
  • Other single or multiple agent assays (e.g., flow cytometry)
  • Cell Line verification (STR analysis)
  • Mycoplasma testing
• Pharmacokinetic assay performance
  • Quantitative measurement of drugs, metabolites, and other small molecules
  • Pharmacokinetic data analysis

Resources

Translational Research Laboratory

• Nanodrop 2.0 spectrophotometer
• Qubit 2.0 Fluorometer
• Leica phase contrast inverted microscope
• Comet analysis electrophoresis system & Komet version 5.5 analysis Software
• Refrigerated centrifuges & microcentrifuges
• -70 C Freezers with integrated alarm systems
• Laminar flow hoods and CO2 Tissue Culture Incubators
• SpectraMax i3x

Pharmacology Laboratory

• Applied Biosystems API 3200 triple quadrupole tandem mass spectrometer
• Shimadzu LC-20 series HPLC system
• SPD-M20A photodiode array/RF-10AxL fluorescence detectors
• SIL-20AC refrigerated autosampler
• Agilent 6540 accurate-mass quadrupole time-of-flight (Q-TOF) mass spectrometer
• Agilent 1290 ultra-high performance liquid chromatography (UPLC) system
• Photodiode array (PDA) detector
• Equipment for biological sample preparation and storage
• Computers and data storage devices
• Software for pharmacokinetic and pharmacodynamic analyses

Fees

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Division: Preclinical

The Athymic Animal & Xenograft Core consists of two physical sites, one at °Ç¸çºÚÁÏ (CWRU) and the other at Cleveland Clinic's Taussig Cancer Institute. The purpose of the facility is to help principal investigators of CWRU and Cleveland Clinic perform tumor experiments, angiogenesis studies, and pharmacokinetic studies in mice. The facility routinely conducts animal experiments involving several syngeneic murine tumor models and several human xenograft (athymic nude mouse and NSG–NOD scid IL2Rg mouse) tumor models. In addition, the facility has developed several orthotopic tumor models: brain, lung, breast, renal, spleen, skin, ovarian, prostate, bladder, and pancreas. The facility is always willing to develop additional new models.

Services

The Athymic Animal & Xenograft Core utilizes murine models to facilitate in vivo cancer drug screening, drug evaluation, and drug development. Services include drug administration (sub-cutaneous, intravenous, intraperitoneal, prolonged slow-release silastic capsule), blood drawing (repetitive - saphenous vein, or terminal - cardiac puncture) for pharmacokinetic pharmacodynamic (PK/PD) studies, absorption distribution metabolism excretion (ADME) studies, syngeneic and xenograft tumor models, orthotopic tumor models. Services provided help in evaluation of new pharmacologic agents by providing data on tumor growth, metastasis, angiogenesis, and gene expression. The facility also provides consultation services to assist the investigator in all aspects of animal tumor model development.

Resources

• Laminar flow workstation
• Laboratory work room containing two laminar flow hoods and a type B, 100% exhaust biohazard hood to accommodate preparation of cancer cells, anticancer agents, and other pharmaceutical agents
• Full complement of stainless steel surgical instruments
• Pass-through autoclave connecting the three animal rooms
• Steris "super-autoclave" installed adjacent to the facility as a backup

Fees

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